Journal: International Journal of Molecular Sciences
Article Title: Agathisflavone Modulates the Kynurenine Pathway and Glial Inflammatory Responses with Implications for Neuroprotection
doi: 10.3390/ijms262411951
Figure Lengend Snippet: Neuroprotective effects of astrocyte-derived conditioned medium (ACM) on differentiated PC12 cells. ( A ) Representative phase-contrast images showing PC12 cell morphology. ( B ) MTT assay showing viability of PC12 cells after exposure to ACM from astrocytes treated with LPS, QUIN, 1-MT, FAB, or combinations. Data represent mean ± SD ( n = 3 independent experiments). Scale bar: 50 μm. Statistical analysis: one-way ANOVA followed by Bonferroni’s test; #### p < 0.0001 vs. the respective stimulus without FAB.
Article Snippet: PC12 neuronal cells, derived from a murine adrenal pheochromocytoma was purchased from American Type Culture Collection (ATCC) (#CRL-1721.1 PC12 ADH, Rattus norvegicus, Manassas, VA, USA), serve as an in vitro neuronal model due to their capacity to extend neurites and exit the cell cycle upon stimulation with growth factors such as nerve growth factor (NGF) and fibroblast growth factor 1 (FGF1) [ , ].
Techniques: Derivative Assay, MTT Assay